Proliferation deficits and gene expression dysregulation in Down's syndrome (Ts1Cje) neural progenitor cells cultured from neurospheres
Identifieur interne : 000126 ( France/Analysis ); précédent : 000125; suivant : 000127Proliferation deficits and gene expression dysregulation in Down's syndrome (Ts1Cje) neural progenitor cells cultured from neurospheres
Auteurs : Randal X. Moldrich [France, Australie] ; Luce Dauphinot [France] ; Julien Laffaire [France] ; Tania Vitalis [France] ; Yann Hérault [France] ; Philip M. Beart [Australie] ; Jean Rossier [France] ; Denis Vivien [France] ; Corinne Gehrig [Suisse] ; Stylianos E. Antonarakis [Suisse] ; Robert Lyle [Suisse] ; Marie-Claude Potier [France]Source :
- Journal of Neuroscience Research [ 0360-4012 ] ; 2009-11-01.
English descriptors
- KwdEn :
Abstract
Down's syndrome neurophenotypes are characterized by mental retardation and a decreased brain volume. To identify whether deficits in proliferation could be responsible for this phenotype, neural progenitor cells were isolated from the developing E14 neocortex of Down's syndrome partial trisomy Ts1Cje mice and euploid (WT) littermates and grown as neurospheres. Ts1Cje neural progenitors proliferated at a slower rate, because of a longer cell cycle, and a greater number of cells were positive for glial fibrillary acidic protein. An increase in cell death was also noted. Gene expression profiles of neural progenitor cells from Ts1Cje and WT showed that 54% of triploid genes had expression ratios (Ts1Cje/WT) significantly greater than the expected diploid gene ratio of 1.0. Some diploid genes associated with proliferation, differentiation, and glial function were dysregulated. Interestingly, proliferation and gene expression dysregulation detected in the Ts1Cje mice did not require overexpression of the chromosome 21 genes amyloid precursor protein (App) and soluble superoxide dismutase 1 (Sod1). © 2009 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jnr.22131
Affiliations:
- Australie, France, Suisse
- Centre-Val de Loire, Région Centre, Île-de-France
- Caen, Orléans, Paris
- Université d'Orléans
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Links to Exploration step
ISTEX:7872C596FE9257F4EAAB21BEF279D92CDF0103A4Le document en format XML
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Antonarakis</name><affiliation wicri:level="1"><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Genetic Medicine and Development, University of Geneva Medical School and University Hospitals of Geneva, Geneva</wicri:regionArea><wicri:noRegion>Geneva</wicri:noRegion></affiliation></author><author><name sortKey="Lyle, Robert" sort="Lyle, Robert" uniqKey="Lyle R" first="Robert" last="Lyle">Robert Lyle</name><affiliation wicri:level="1"><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Genetic Medicine and Development, University of Geneva Medical School and University Hospitals of Geneva, Geneva</wicri:regionArea><wicri:noRegion>Geneva</wicri:noRegion></affiliation></author><author><name sortKey="Potier, Marie Laude" sort="Potier, Marie Laude" uniqKey="Potier M" first="Marie-Claude" last="Potier">Marie-Claude Potier</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Neurobiologie et Diversité Cellulaire, CNRS UMR7637, ESPCI, Paris</wicri:regionArea><placeName><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Current Address: CRICM CNRS UMR7225 INSERM UMR975 UPMC, Hôpital de la Pitié Salpétrière, 47 Bd. de l'Hôpital, 75013 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Neuroscience Research</title><title level="j" type="abbrev">J. Neurosci. Res.</title><idno type="ISSN">0360-4012</idno><idno type="eISSN">1097-4547</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2009-11-01">2009-11-01</date><biblScope unit="volume">87</biblScope><biblScope unit="issue">14</biblScope><biblScope unit="page" from="3143">3143</biblScope><biblScope unit="page" to="3152">3152</biblScope></imprint><idno type="ISSN">0360-4012</idno></series><idno type="istex">7872C596FE9257F4EAAB21BEF279D92CDF0103A4</idno><idno type="DOI">10.1002/jnr.22131</idno><idno type="ArticleID">JNR22131</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0360-4012</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Down's syndrome</term><term>Ts1Cje</term><term>gene expression</term><term>neocortex</term><term>neural progenitor cells</term><term>neurosphere</term><term>proliferation</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Down's syndrome neurophenotypes are characterized by mental retardation and a decreased brain volume. To identify whether deficits in proliferation could be responsible for this phenotype, neural progenitor cells were isolated from the developing E14 neocortex of Down's syndrome partial trisomy Ts1Cje mice and euploid (WT) littermates and grown as neurospheres. Ts1Cje neural progenitors proliferated at a slower rate, because of a longer cell cycle, and a greater number of cells were positive for glial fibrillary acidic protein. An increase in cell death was also noted. Gene expression profiles of neural progenitor cells from Ts1Cje and WT showed that 54% of triploid genes had expression ratios (Ts1Cje/WT) significantly greater than the expected diploid gene ratio of 1.0. Some diploid genes associated with proliferation, differentiation, and glial function were dysregulated. Interestingly, proliferation and gene expression dysregulation detected in the Ts1Cje mice did not require overexpression of the chromosome 21 genes amyloid precursor protein (App) and soluble superoxide dismutase 1 (Sod1). © 2009 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li><li>Suisse</li></country><region><li>Centre-Val de Loire</li><li>Région Centre</li><li>Île-de-France</li></region><settlement><li>Caen</li><li>Orléans</li><li>Paris</li></settlement><orgName><li>Université d'Orléans</li></orgName></list><tree><country name="France"><noRegion><name sortKey="Moldrich, Randal X" sort="Moldrich, Randal X" uniqKey="Moldrich R" first="Randal X." last="Moldrich">Randal X. Moldrich</name></noRegion><name sortKey="Dauphinot, Luce" sort="Dauphinot, Luce" uniqKey="Dauphinot L" first="Luce" last="Dauphinot">Luce Dauphinot</name><name sortKey="Dauphinot, Luce" sort="Dauphinot, Luce" uniqKey="Dauphinot L" first="Luce" last="Dauphinot">Luce Dauphinot</name><name sortKey="Herault, Yann" sort="Herault, Yann" uniqKey="Herault Y" first="Yann" last="Hérault">Yann Hérault</name><name sortKey="Laffaire, Julien" sort="Laffaire, Julien" uniqKey="Laffaire J" first="Julien" last="Laffaire">Julien Laffaire</name><name sortKey="Laffaire, Julien" sort="Laffaire, Julien" uniqKey="Laffaire J" first="Julien" last="Laffaire">Julien Laffaire</name><name sortKey="Potier, Marie Laude" sort="Potier, Marie Laude" uniqKey="Potier M" first="Marie-Claude" last="Potier">Marie-Claude Potier</name><name sortKey="Potier, Marie Laude" sort="Potier, Marie Laude" uniqKey="Potier M" first="Marie-Claude" last="Potier">Marie-Claude Potier</name><name sortKey="Rossier, Jean" sort="Rossier, Jean" uniqKey="Rossier J" first="Jean" last="Rossier">Jean Rossier</name><name sortKey="Vitalis, Tania" sort="Vitalis, Tania" uniqKey="Vitalis T" first="Tania" last="Vitalis">Tania Vitalis</name><name sortKey="Vivien, Denis" sort="Vivien, Denis" uniqKey="Vivien D" first="Denis" last="Vivien">Denis Vivien</name></country><country name="Australie"><noRegion><name sortKey="Moldrich, Randal X" sort="Moldrich, Randal X" uniqKey="Moldrich R" first="Randal X." last="Moldrich">Randal X. Moldrich</name></noRegion><name sortKey="Beart, Philip M" sort="Beart, Philip M" uniqKey="Beart P" first="Philip M." last="Beart">Philip M. Beart</name></country><country name="Suisse"><noRegion><name sortKey="Gehrig, Corinne" sort="Gehrig, Corinne" uniqKey="Gehrig C" first="Corinne" last="Gehrig">Corinne Gehrig</name></noRegion><name sortKey="Antonarakis, Stylianos E" sort="Antonarakis, Stylianos E" uniqKey="Antonarakis S" first="Stylianos E." last="Antonarakis">Stylianos E. Antonarakis</name><name sortKey="Lyle, Robert" sort="Lyle, Robert" uniqKey="Lyle R" first="Robert" last="Lyle">Robert Lyle</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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